Comparison between Measurements of Time-resolved Autofluorescence of the Fundus and In-vitro Measurements of Substances
Schweitzer D.1, Hammer M.1, Gaillard E.2
1Experimental Ophthalmology, University Eye Clinic Jena, 2Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb/USA
Purpose: Pathologic alterations of metabolism are discussed as a reason for the development of age-related macular degeneration. For proof of such alterations, the 2-dimensional detection of time-resolved autofluorescence was developed.
Method: It was the goal of the study to compare first in vivo measurements of time-resolved autofluorescence with measurements of isolated substances. Both in vivo and in vitro measurements were performed using the Jena Tau Mapper. In this device, the fluorescence is excited in a 20° field by laser pulses of 100 ps duration at 446 nm. The emission is detected above 475 nm. The fluorescence lifetime was determined in bi-exponential approximation for reduced nicotinamid-adenindinucleotide (NADH), flavinadenindinucleotide (FAD), N-retinyl-N-retinylidene-ethanolamine (A2E), human lipofuscin, and advanced glycation end-product (AGE).
Results: Drawing the results in tau 1 - tau 2 diagrams, substance specific clusters were exhibited. The in vivo measurements result also in clusters, depending on the fundus morphology. An influence of the
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