Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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Effect Of PEDF on the Proliferation of Bovine Retinal Pigment Epithelial Cells and Pericytes

Baldysiak-Figiel A., Lang G. K., Lang G. E.
Department of Ophthalmology, University Eye Hospital, Ulm

Purpose: Diabetes mellitus and age-related macular degeneration (AMD) account for major causes of blindness in industrialized countries. In diabetic retinopathy, the proliferation of retinal endothelial cells is induced in part by progressive loss of pericytes, which is associated with the lack of the negative growth factor TGFß2. In AMD, the proliferation of choroidal endothelial cells is evoked by growth factor overproduction originating from proliferating retinal pigment epithelial cells (RPE). In this study we investigated the effect of PEDF, a potent serin protease inhibitor on RPE and pericytes.
Method: Bovine retinal pigment epithelial cells (BRPE) und pericytes were isolated and cultured in DMEM+10% FCS. Confluent 96-well plates with cells were starved in serum-free medium. After 24 h the cells were incubated with PEDF (0.1-100 ng/ml) in serum free medium containing [3H]-thymidine for another 24 h. Statistical analysis was performed using t-test.
Results: PEDF showed different effects on BRPE and on pericyte proliferation. PEDF directly inhibited the proliferation of BRPE at a concentration of 1 ng/ml (p<0.0002). The proliferation of pericytes significantly increased in the presence of PEDF at the concentration of 10 ng/ml (p<0.0001).
Conclusions: Our data show that PEDF has different concentration-dependent effects. It is able to inhibit the prol

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