Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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Corneal Endothelial Transplantation Using Femtosecond Laser Technology

Voßmerbäumer U., Jonas J. B.
Dept of Ophthalmology, Faculty for clinical medicine Mannheim, University of Heidelberg

Purpose: Experimental case report to describe a technique of corneal endothelial transplantation using femtosecond laser technology.
Method: Using a femtosecond laser, a thin deep corneal layer including Descemet´s membrane and corneal endothelium was prepared with a diameter of 6 mm on its endothelial side, and a diameter of 8 mm on its stromal side. On top of the deep corneal lamella, a thick corneal flap with three positional pikes and a hinge was created in 5 freshly enucleated porcine eye. After opening the flap, the deep corneal layer with Descemet´s membrane and corneal endothelium was removed and inserted into the bed of recipient eyes which had been prepared in the same way.
Results: For all eyes included in the study, the deep corneal grafts and the flaps could be prepared without major difficulties. The deep lamellar allografts could easily be transplanted into the recipient beds of other eyes. They were eventually covered by the autologous corneal flap of the recipient eye. The time taken for the preparation of the deep corneal grafts and the flaps was less than 5 minutes in all cases.
Conclusions: Femtosecond laser technology may allow non-mechanical posterior lamellar keratoplasty with transplantation of Descemet´s membrane and corneal endothelium, leaving most of the corneal stroma and corneal surface untouched.

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