Effects of Verapamil-isomers on the Proliferation of Choroidal Endothelial Cells and Retinal Pigmented Epithelial Cells
Hoffmann S., Friedrichs U., Eichler W., Balthasar S., Wiedemann P.
Department of Ophthalmology, University of Leipzig
Purpose: The exsudative age-related macular degeneration (AMD) is a common cause of blindness of the elderly. Neovascular membranes are formed by the interaction of choroidal endothelial cells (CECs) and retinal pigmented epithelial cells (RPEs). In this study, we investigated the effects of the Verapamil isomers D, L and D/L on the serum induced proliferation of CECs and RPE-cells.
Method: Bovine CECs and RPE cells were treated for a time course of 5 days with the different Verapamil isomers in a concentration range of 0.1 to 100 µM and 10 % fetal bovine serum. After the incubation period, proliferation was estimated by an MTT assay and cell counting. The cell viability after verapamil exposition was determined by trypan blue exclusion.
Results: The Verapamil isomers are inhibiting the proliferation of CECs and RPE cells in a concentration range of 10 to 100 mM. No toxicity was seen in a concentration range of 50 mM Verapamil and lower by trypan blue exclusion. The Verapamil D-isomer effecting protein kinase C showed similar effects in comparison to the L-isomer or the D/L isomer.
Conclusions: Verapamil isomers are capable of inhibiting the proliferation of CECs and RPE cells. The D-isomer only affects the protein kinase C without effects on c
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