Inhibitory Effect of Certain Neuropeptides on the Proliferation of Human Retinal Pigment Epithelial Cells
Troger J., Sellemond S., Kieselbach G., Kralinger M., Schmid E., Teuchner B., Nguyen Q. A., Schretter-Irschick E., Göttinger W.
Dept. of Ophthalmology, Innsbruck University
Purpose: To define the effect of substance P, calcitonin gene-related peptide, vasoactive intestinal polypeptide, neuropeptide Y and secretoneurin on the proliferation of human retinal pigment epithelial cells.
Method: ARPE-19 cells were used. The cells were standardly cultured in DMEM medium and one thousand and two thousand cells were incubated with the peptides for three and five days. Estimation of the proliferation was evaluated by an ATP lite assay. Furthermore highly specific antagonists were used to find out whether the peptide effect would be reversed.
Results: Each peptide featured an inhibitory effect on the proliferation. This inhibitory effect was weak averaging 10% to 15%, but was highly significant and most pronouncedly seen at low concentrations between 10-10M and 10-14M. Each antagonist reversed the effect.
Conclusions: The results clearly show that retinal pigment epithelial cells are under neural control and these neuropeptides constitute the first endogenous inhibitors of retinal pigment epithelial cell proliferation. For SN, this is the first biological effect in the eye. The results are relevant both physiologically and pathophysiologically. Physiologically, the inhibitory effect may mean that neuropeptides cause the cells to maintain in
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