Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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A Surgical Technique for Chronical Implantation of Epiretinal Implants in Cats

Rodrigues E. B., Hesse L., Schanze T. 
NeuroPhysics Group, Philipps-Universität Marburg

Purpose: Retina implants are by several groups world-wide researched. So far there is no standard surgical technique or animal model for chronicle epiretinal implants (EI) research. This study aims to present a surgical technique for chronicle implantation and fixation of active EI in cats.
Method: Before intraocular implantation, we tested in-vitro the effect of either balanced salt solution (BSS), perfluorocarbon liquid (PFC), or silicone oil (SO) on the electrode array (EA) conduction, as well as the cleaning of those liquids out of the EA. Five cats were operated (n=5, 3-5Kg), and followed clinically by an average of five months. Phacoemulsification followed by pars plana vitrectomy (PPV) were performed. All incisions were made through the limbus. Indocyanine green (ICG) was intravitreally injected for better visualization of the vitreous remnants. The electrode array was positioned on the retina, and PFC was injected on the electrode array in three eyes. PFC (n=2) was slowly removed to releasing the flexible part of the EI. In two animals scleral fixation of the EI was performed.
Results: SO and PFC do not allow electric conduction of EA. When the EA was positioned in contact to the retina (in-vitro), no liquid disturbed the electric conduction. If the EA was shortly removed away from the retina, SO attached to the EA surface. Then, electrical conduction was not

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