Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

„To Stain or not to Stain“: Vital Dyes in Macular Surgery

Grisanti S.
Eye Hospital (Dept. I) of the Eberhard-Karls University Tübingen

Internal limiting membrane (ILM) removal is becoming an important step in the treatment of macular holes and chronic cystoid macular edema. Due to poor visibility of the ILM, however, the complete removal is difficult and not always obvious. The introduction of the dyes indocyanine green and trypan blue has improved the routine and meticulous removal of the ILM tremendously. The staining relies on the dyes affinity to basement membrane molecules, such as laminin. The unresolved questions, however, are whether the dyes have also a disruptive effect on cells and cell junctions and whether retinotoxicity can be excluded. Based on the own experience and the literature the advantages and disadvantages of a dye assisted ILM peeling will be discussed.


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