Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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Alkylphosphocholines Inhibit Proliferation and Contraction of Human Retinal Pigment Epithelial Cells

Eibl K. H., Schoenfeld C., Neubauer A. S., Priglinger S., Kampik A., Welge-Lüßen U.
Department of Ophthalmology, University Eye Hospital, Ludwig-Maximillian-University, Munich

Purpose: Proliferative Vitreoretinopathie (PVR) is a major complication in retinal detachment surgery. Proliferation and contraction of retinal pigment epithelial cells (RPE) in the vitreous seems to be fundamental in PVR membrane formation and subsequent retinal detachment. So far, there is no treatment in routine clinical use to prevent this condition. The aim of our study was to test Alkylphosphocholines (APCs) for their antiproliferative and anticontractile effect on human RPE cells in vitro and to elucidate their mechanism of action.
Method: Cultured RPE cells of five human donors were treated over 24 hrs. with one of four APCs (C18:1-PC, C20:1-PC, C21:1-PC and C22:1-PC) in the presence of fetal calf serum. Proliferation was assessed by the tetrazolium dye-reduction assay (MTT). The effect of APCs on RPE mediated matrix contraction was determined by cell-seeded three-dimensional collagen gels. Cell viability was tested by the trypan blue exclusion assay. PKC activity was measured by a radioactive assay.
Results: All four APCs are able to inhibit RPE proliferation and RPE mediated collagen matrix contraction in vitro. The antiproliferative and anticontractile effect of APCs increases in a dose-dependent manner. The IC50 concentrations for each APC are given (C18:1-PC 26.

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