Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Two-photon Excitated Fluorescence in Ocular Melanomas - First Results on the Way to a New Diagnostic Method

Schneider M.1, Leupold D.2
1Department of Ophthalmology, Philipps University, Marburg, 2Max-Born Institute for Non-linear Optics and Short Pulse Spectroscopy, Berlin

Purpose: Ocular melanomas still cause diagnostic problems. The decision for therapy is most often made on the basis of clinical examination and ultrasound. The aim of our project is the development of a new diagnostic method, which allows a non-invasive differentiation between ocular melanomas and benign melanocytic tissue.
Method: Tissue samples of benign melanin-containing tissue and melanomas were taken to detect the fluorescence of melanin by stepwise two-photon excitation with femtosecond pulses at 800nm. This is the basic methodological difference to the long-time known one-photon fluorescence at 400nm. The fluorescence spectrum of melanin was investigated by using synthetical melanin.
Results: Eight tumours were examined (melanoma and benign lesions, pigment epithelium) and the results were also compared to the fluorescence spectra of cutaneous melanin-containing tumours. Conventional 400nm one-photon excitated fluorescence showed a maximum at 480nm with no differences between the samples. In contrast, two-photon femtosecond excitation revealed a specific melanin fluorescence of the long-wavelength part. The conjunctival melanoma showed a clear shift to the red part of the spectrum at fluorescence maximum as well as an increased intensity of the


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