|Programm||"Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"|
High Efficiency Gene Electrotransfer to the Endothelium of Human Organ Cultured Corneas
Thuret G.1, Manissolle C.1, Bonnefoy R.1, Campos-Guyotat L.1, Maugery J.1, Gain P.1
Purpose: Gene transfer to the corneal endothelium is a hopeful strategy to modulate cell functions through the induction of newly synthetised proteins with therapeutic purpose. Gene electrotransfer is deemed safer and triggers dramatically less inflammation than viral vectors and is also reputed to be more efficient than other non viral vectors. Our aim was to develop and optimise ex vivo gene electrotransfer to endothelial cells (ECs) of organ cultured human corneas.