Immunohistopathologic Evaluation of Proliferative Activity in Choroidal Neovascularization after PDT
Tatar O.1, Grisanti S.1, Canbek S.1, Lafaut B.2, Gelisken F.1, Bartz-Schmidt K. U.1
1Center of Ophthalmology, Department of Vitreoretinal Surgery Eberhard-Karls University Tuebingen; 2University of Ghent / AZ St. Jan, Bruges/B
Purpose: To evaluate the proliferative activity of classic choroidal neovascular membranes (CNVM) secondary to age related macular degeneration (ARMD) excised at different times (3 days- 5 months) after photodynamic therapy (PDT).
Method: Seven subfoveal classic CNVM, excised between 3 days and 5 months after PDT, were stained for CD34 (panendothelial marker), CD105 (proliferation marker for endothelial cells), and Ki-67 (nuclear marker of proliferation) and compared to the clinical course.
Results: Fluorescein angiography (FA) performed on the day of surgery disclosed non-perfusion of the treated area 3 days after PDT, but reperfusion and leakage was seen at greater post-PDT intervals. Immunohistopathologic examination of 4 CNVM excised 3 days after PDT disclosed several vessels that were positive for CD34 and CD105. The endothelial cells appeared damaged, but several of the lumina were not occluded. Ki-67 expression was prominent only in one specimen. Within this specimen the nonoccluded vessels were predominant. CNVM excised 1 to 5 months after PDT disclosed many patent vessels lined by healthy endothelial cells with strong expr
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