Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

   Hotel Registration
   Welcome address
Beteiligte Gesellschaften
   Societies involved
DOG Information
   DOG Information
Eröffnung des Kongresses
   Opening Ceremony
Ablauf der Tagung 2003
   General overview of congress
Lageplan der Räumlichkeiten
   Map of Congress Center
Wissenschaftliche Themen
   Scientific topics
Wissenschaftliches Programm
   Scientific program
   Poster Presentation
Begleitende Veranstaltungen
   Accompanying program
   Working sessions
   Social program
Allgemeine Informationen
   General Information
   Index of Authors
   Commercial exhibitors

DOG Homepage


Dexlipotam and Formation of AGEs in RPE-cells

Akkoyun I., Lin J., Feng J., Jonas J. B., Hammes H. P.  
Universitäts-Augenklinik, V. Med. Klinik, Universitätsklinikum Mannheim der Universität Heidelberg, Mannheim

Purpose: Dexlipotam has been designed as drug for the treatment of diabetic polyneuropathy and vascular dysfunction. To investigate the effect of dexlipotam on advanced glycation endproducts (AGE’s) in human retinal pigment epithelium (RPE) cells regarding the possible role of oxidative stress in the pathogenesis in age-related macular degeneration (AMD).
Method: Cell cultures of the human RPE-K1034 cell line were incubated with various concentrations of dexlipotam (0.0, 1.0, 3.0, 10.0 mM) for 1 and 6 hrs, respectively, under baseline conditions (5 mM glucose), in a medium containing 5mM glucose + 30 mM H2O2 for 1 hr, and in a medium containing 25mM glucose. Westernblot analysis was performed to detect N-carboxymethyl-lysine (CML) as a marker of AGEs/glycoxidation.
Results: Both, under baseline conditions, and upon additional exposure to 30 mM H2O2  for 1 hr, a ~80 kD CML-modified protein band was present. Co-incubation with increasing concentrations of dexlipotam up to 10.0 mM did not alter this modification. Unexpectedly, this modification was not observed when cells were incubated in high glucose for 72 hrs. However, adding dexlipotam (1.0, 3.0, 10.0 mM) induced a stronger CML modification of the ~80 kD protein band. Longer exposure to dexlipotam (6 hrs, 1.0 and

Zurück | Back