Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Identification of Glutathione-stransferase in Human Cataractous Lenses

Dawczynski J.1, Lupp A.2, Königsdörffer E.1, Augsten R.1, Blum M.3, Müller D.2, Strobel J.1
1Dept. of Ophthalmology, 2Dept of Pharmacology, University Hospital Jena; 3Dept. of Ophthalmology, HELIOS-Hospital Erfurt

Purpose: Glutathione-S-transferase (GST) might be one enzyme associated in antioxidative mechanism of human lenses.this study aimed to investigate the GST content of human lenses in different forms of cataract. Furthermore, the possible influence of diabetes was investigated.
Method: 58 human lenses with a mean age of 67.7 years (n=22 corticonuclear cataract, 36 mature cataract;10 diabetic) were investigated.GST activity was measured spectophotometrically by coupling of 1-chloro-2,4-dinitrobenzene (CDNB) or o-dinitrobenzol to glutathione to the method described by Habig.Statistics was performed by Mann-Whitney Test.
Results: Mature cataracts showed a significant loss of GST-activity compared to corticonuclear cataracts (o-dinitrobenzol: 0.092±0.009 vs. 0.087±0.007 nmol/min, p=0.004; CDNB: 49.10±6.14 vs. 45.93±4.26 nmol DNB-GS/min, p=0.06). Dark brown lenses found to be signicicant lowered in GST activity compared to light-brown lenses (o-dinitrobenzol: p=0.005; CDNB: p=0.018). However, diabetic lenses showed very similar GST-activity compared to non-diabetic lenses (o-dinitrobenzol: 0.090±0,009 vs. 0.090±0.004 nmol/min; CDNB: 48.44±5.88 vs. 45.43±3.14 nmol DNB-GS/min).
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