Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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Pathogenesis of Vitreoretinal Membranes in Mice with Targeted Disruption of the Norrie Gene

Fuchs A. V.1, Adamek E.2, Ohlmann A.2, Kampik A.1, Lütjen-Drecoll E.2
1Department of Ophthalmology, Ludwig Maximillian University, Munich; 2Dept. of Anatomie II, Friedrich-Alexander University, Erlangen

Purpose: In patients with Norrie disease (ND) and in gene targeted ND mutant mice retinal degeneration associated with impaired capillarisation as well as membranous lesions of unknown origin within the vitreous have been described. Previous studies suggested persisting hyaloid vessels (HV) as major component of these membranes. Endostatin, an angiogenetic inhibitor is known to play a role for the regression of HV during ocular development. The purpose of this study was to further characterise these membranes in ND mice and to assess the role of endostatin for their formation.
Method: Retinal wholemounts of ND mice aged P0, P5, P9, P12, P21, 3 months and one year as well as age matched controls were single and double stained with NADPH diaphorase, smooth muscle alpha actin and endostatin. Futhermore immunoreactivity for endostatin was studied on sections by light- and electronmicroscopy methods, and Western blot analyses.
Results: In ND mice hyaloid vessels do not regress in contrast to age matched controls. In HV of controls and in persisting HV of ND mice there was no difference in endostatin staining and distribution. In both groups it was localised in the outer basemen

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