Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Corneal Epitheliotrophic Capacity of Serum from Immunosuppressed Donors versus Healthy Individuals

Harloff S., Hartwig D., Dibbelt L., Wedel T., Geerling G.
University Clinical Centre Schleswig-Holstein, Luebeck

Purpose: Autologous serum has been advocated for the treatement of persistent corneal epithelial defects and other ocular surface disorders which may be a local manifestation of systemic disease, such as rheumatoid arthritis or ocular cicatricial pemphigoid. Many of these underlying disorders are cytokine mediated and require immunosuppressive therapy, which could potentially have an influence on the epitheliotrophic capacity of serum eyedrops. We compared supportive effect of serum from healthy and immunosuppressed donors in a human corneal epithelial cell culture model.
Method: Serum was prepared from full blood samples of 10 healthy donors and 10 patients suffering from rheumatoid arthritis and treated with prednisolone, methotrexate and azathioprine under standardised clotting and centrifugation conditions. In these samples EGF, FGF, HGF, PDGF-AB, TGF-b1, Fibronectin, Vitamine A and E as well as TNF-a and IL-6 were quantified by means of routine ELISA- or HPLC-technology. SV-40 immortalised human corneal keratinozytes were cultured in 96-well plates at 37°C, 5% CO2 with a fully defined culture medi


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