Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

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Trophic Capacity of Platelet Lysate on Corneal Epithelial Cells – An Alternative to Autologous Serum Eye Drops for Ocular Surface Disease?

Geerling G., Harloff S., Liu L., Wedel T., Hartwig D.
Dept. of Ophthalmology, Medical University, Luebeck

Purpose: Eye drops prepared from serum have been successfully used to treat ocular surface disorders such as dry eyes or persistent epithelial defects. Their beneficial effect was attributed to a variety of growth factors present in serum. Platelets are a major source of growth factors in serum. They are available at adjustable concentrations in platelet rich suspensions from blood banks and – following stimulation with thrombin – release their growth factor content. We examined the epitheliotrophic capactiy of platelet lysates in comparison with serum in an in-vitro model of human corneal epithelial cells.
Method: Platelet concentrates with 4 x 109 thrombocytes / ml and full blood samples were obtained of 10 healthy donors each and prepared under standardised clotting and centrifugation conditions. In these EGF, HGF, FGF PDGF, TGF-b, fibronectin, as well as vitamin A and E were quantified by means of routine ELISA- or HPLC-technology. SV-40 immortalised human corneal keratinocytes were cultured in 96-well plates at 37°C, 5% CO2 with a fully defined culture medium. At 30% confluency the culture medium was substituted by one of the test preparations. Proliferation of cell cultures was quantified by means of a luminescence-based ATP-Assay in dose-response experiments. A colony dis

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