Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Ballistic CTLA4 and IL-4 Gene Transfer into the Lower Lid Prolongs Orthotopic Corneal Graft Survival in Mice

Hoffmann F.1, Zhang E.-P.1, Franke J.2, Schroff M.3, Junghans C.3, Wittig B.4
1Department of Ophthalmology and 2Nuclear Medicine, University Hospital Benjamin Franklin (UKBF), 4Institute for Molecular Biology and Bioinformatics, Free University of Berlin
3Mologen AG, Berlin

Purpose: To explore outflow from the eye and to determine and modulate the influence of lymphatic drainage on corneal graft survival in mice
Method: Tracer experiments were conducted in BALB/c mice using the 99mTC colloidal albumin Nanocoll®. Count rates were determined in the eyes, submandibular lymph nodes, spleen, liver and blood 24 hours after subconjunctival, intracorneal, intracameral (anterior chamber), intravenous and subcutaneous lower-lid or upper-lid injections (n=6 each). Three groups of mice underwent submandibular lymphadenectomy either on the left 7 or 21 days prior to Nanocoll® injection or bilaterally 7 days beforehand. Eight groups of BALB/c mice received corneal transplants from C3H mice; three of them underwent preoperative submandibular lymphadenectomy as mentioned above, three other groups were treated ballistically with vector CTLA4+IL-4 onto the leg or the lower lid.
Results: Radioactivity was detected in the liver, spleen and ipsilateral lymph node as follows: 91.9%, 6.6% and 1.2% respectively after intracameral injection and 26.8%,


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