Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Corneal Substance-P-Ergic Innervation after Laser Epithelial Keratomileusis (LASEK) in Comparison to Photorefractive Keratectomy (PRK) in Rabbits

Tilch D.1, Selbach J. M.1, Laube T.1, Theiss C.2, Steuhl K. P.1, Meller D.1
1Department of Ophthalmology, University of Essen; 2Department of Cytology, Institute of Anatomy, University of Bochum

Purpose: Previous studies have shown that stromal wound healing in PRK is delayed in comparison to LASEK. Corneal afferent nerve fibers deriving from the trigeminal ganglion and with substance P (SP) as neurotransmitter are believed to have trophic function. Therefore, we investigated corneal substance-P-ergic innervation after LASEK and conventional PRK in rabbits.
Method: LASEK and PRK were performed in rabbits and studied after 1, 3, 10 and 20 days (each group n=3-5). In all eyes, hemidesmosomes of corneal epithelium were weakened with 30% ethanol for 3.5 min. In the PRK group, the whole epithelium was removed, but in the LASEK group epithelial flap was repositioned and left to adhere to the underlying stromal bed. Excimer photoablation was performed unilaterally with a 6.0mm ablation zone and 80mm depth equivalent to -6.0 dpt using the SUMMIT APEX PLUS laser with photorefractive keratectomy (PRK) mode. Serial tangential cryosections were collected and labeled with a monoclonal antibody directed against SP. Sections were analyzed by means of laser confocal microscopy.
Results: One day after LASEK, eyes showed


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