Photodynamic Therapy of Ciliary Body in Experimental Glaucoma
Hafezi-Moghadam N.1,2, Ahmed E.1,Gragoudas E.1,2, Miller J W.1,2
1Angiogenesis Laboratory, 2 Retina Service, Massachusetts Eye and Ear Infirmary, Boston, MA
Purpose: Cyclophotocoagulation is utilized in refractory glaucoma but complications include vision loss, hypotony and phthisis. Treatment of the ciliary body using photodynamic therapy (PDT) with verteporfin may be an effective and safer treatment for reduction of intraocular pressure (IOP).
Method: Three vein cautery (3VC) was performed in both eyes of Brown Norway rats to create elevated IOP as a model of glaucoma, modified from Shareef et al. 1995. The IOP was measured on conscious animals with the Tono-Pen XL by a masked investigator prior to 3VC and in follow-up on days 1, 7, and 14. Each IOP value represents the average of five measurements. One eye from each of the seven 3VC animals was randomly assigned to PDT, while the fellow eye served as a control. Contact transscleral verteporfin PDT was performed using 2 mg/kg intravenous verteporfin, and 689 nm light (600 mm spot, 600 mW/cm2, 150 J/cm2) administered via a fiberoptic probe placed posterior to the limbus to cover 90 degrees of the ciliary body. IOP was measured on both eyes 1 day and at weekly intervals for up to 28 days after PDT. The paired t-Test for two-sample assuming equal variances was used to determine differences in mean IOP between PDT-treated and untreated eyes.
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