Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"


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Abstract
Abstract

Effects of CD95L and Daunomycin on Retinal Function in Superfused Vertebrate Retina

Lüke M.1, Lüke C.1, Hueber A1, Walter P.1, Kirchhof B.1, Sickel W.2
1
Dept. of Ophthalmology, 2Institute for Neurophysiology, Cologne University

Purpose: CD95L is a cytokine that triggers an intracellular cascade through CD95 receptor which mediates apoptosis in target cells. The combination of daunomycin and CD95L synergistically induces death of retinal pigment epithelial cells. Therefore, intravitreal application of daunomycin combined with CD95L could provide a therapeutic alternative for the treatment of proliferative vitreoretinopathy (PVR). In the present study we investigated the effects of daunomycin with and without CD95L on bovine retinal function.
Method: Bovine retina preparations were perfused with an oxygen preequilibrated standard solution. The electroretinogram (ERG) was recorded using AgAgCl electrodes. After recording of stable ERG amplitudes daunomycin (d) or daunomycin + CD95L (d+CD95L) was added to the solution for 45 minutes. Thereupon the preparation was reperfused with standard solution for 100 minutes. The percentage of b-wave reduction after exposition was calculated.
Results: A b-wave amplitude reduction was found for the following concentrations:
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