Programm                 "Degeneration und Regeneration– Grundlagen, Diagnostik und Therapie"

   Hotel Registration
   Welcome address
Beteiligte Gesellschaften
   Societies involved
DOG Information
   DOG Information
Eröffnung des Kongresses
   Opening Ceremony
Ablauf der Tagung 2003
   General overview of congress
Lageplan der Räumlichkeiten
   Map of Congress Center
Wissenschaftliche Themen
   Scientific topics
Wissenschaftliches Programm
   Scientific program
   Poster Presentation
Begleitende Veranstaltungen
   Accompanying program
   Working sessions
   Social program
Allgemeine Informationen
   General Information
   Index of Authors
   Commercial exhibitors

DOG Homepage


Evidences for Functional Regeneration of the Pathway in Adult Rats

Thanos S. 
Department of Experimental Ophthalmology, Eye Hospital, Münster

Purpose: Mature nerve cell axons do not spontaneously regrow within their natural environment unless they are stimulated by external application of growth-promoting measures. In the present study, axonal regrowth and restoration of visual function was studied in adult rats.
Method: The optic nerve was completely cut, and its proximal and distal stumps were realigned and sutured back together. During the same surgical procedure, the lens was lesioned in order to induce secondary intraocular inflammation, which is known to strongly support the survival of retinal ganglion cells (RGCs) and to promote axonal regeneration within the distal segment of the optic nerve.
Results: The neuroanatomical data showed, that cut axons can regenerate over long distances within the white matter of a central nerve like the adult optic nerve, spanning over 11 mm to the chiasm and between 12 and 15 mm to the thalamus and midbrain. Responsiveness of the pupil to light was restored 5 weeks after injury, thus indicating reinnervation of the pretectal nuclei. Restoration of the ascending pathway between retina and visual cortex was assured by recording flash visual evoked potentials (FVEPs). As expected, no FVEPs could be recorded during the postsurgical period of axonal growth throughout the optic nerve and tract. FVEPs could be recorded after two months indicating that synaptic transmission in higher visual areas was established.

Zurück | Back